PAFAH1B3 Antibody

ProSci
Product Code: PSI-14-759
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-759-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 1
Western blot analysis of extracts of various cell lines, using PAFAH1B3 antibody (14-759) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using PAFAH1B3 antibody (14-759) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
PAFAHG, platelet-activating factor acetylhydrolase IB subunit gamma, PAF acetylhydrolase 29 kDa subunit, PAF-AH 29 kDa subunit, PAF-AH subunit gamma, PAF-AH1b alpha 1 subunit, PAFAH subunit gamma, epididymis secretory sperm binding protein, platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa), platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa)
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with mental retardation, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human PAFAH1B3 (NP_002564.1).
NCBI Gene ID #:
5050
NCBI Official Name:
platelet activating factor acetylhydrolase 1b catalytic subunit 3
NCBI Official Symbol:
PAFAH1B3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q15102
User NOte:
Optimal dilutions for each application to be determined by the researcher.