GCN1L1 Antibody

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ProSci
Product Code: PSI-14-780
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-14-780-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GCN1L1 antibody (14-780) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human colon using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
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Western blot analysis of extracts of various cell lines, using GCN1L1 antibody (14-780) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).

Documents

Further Information

Additional Names:
GCN1LL1, PRIC295, GCN1, eIF-2-alpha kinase activator GCN1, GCN1 (general control of amino-acid synthesis 1, yeast)-like 1, GCN1 eIF-2-alpha kinase activator homolog, GCN1 general control of amino-acid synthesis 1-like 1, GCN1, eIF2 alpha kinase activator homolog, GCN1-like protein 1, general control of amino-acid synthesis 1-like protein 1, hsGCN1, peroxisome proliferator activated receptor interacting complex protein, translational activator GCN1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1).
NCBI Gene ID #:
10985
NCBI Official Name:
GCN1 activator of EIF2AK4
NCBI Official Symbol:
GCN1L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 265kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q92616
User NOte:
Optimal dilutions for each application to be determined by the researcher.