VPS29 Antibody

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ProSci
Product Code: PSI-14-793
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-793-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using VPS29 antibody (14-793) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using VPS29 antibody (14-793) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
VPS29, DC7, DC15, PEP11, FLJ20492, DKFZp564F0223, vacuolar protein sorting 29 (yeast), x 007 protein, retromer protein, vacuolar sorting protein VPS29/PEP11, vacuolar protein sorting 29 (yeast homolog)
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human VPS29 (NP_476528.1).
NCBI Gene ID #:
51699
NCBI Official Name:
vacuolar protein sorting 29 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UBQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.