DNASE1L1 Antibody

ProSci
Product Code: PSI-14-819
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-819-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse heart, using DNASE1L1 Antibody (14-819) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of C6 cells using DNASE1L1 antibody (14-819) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 antibody (14-819) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse heart, using DNASE1L1 Antibody (14-819) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using DNASE1L1 antibody (14-819) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 antibody (14-819) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Deoxyribonuclease-1-like 1, 3121-, DNase X, Deoxyribonuclease I-like 1, DNase I-like 1, Muscle-specific DNase I-like, XIB, DNASE1L1, DNAS1L1, DNL1L
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1).
NCBI Gene ID #:
1774
NCBI Official Name:
Deoxyribonuclease-1-like 1
NCBI Official Symbol:
DNASE1L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49184
User NOte:
Optimal dilutions for each application to be determined by the researcher.