ZNF581 Antibody

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ProSci
Product Code: PSI-14-828
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-828-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using ZNF581 antibody (14-828) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of C6 cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH-3T3 cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U-2 OS cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ZNF581 antibody (14-828) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ZNF581 Polyclonal Antibody (14-828) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ZNF581, FLJ22550, HSPC189
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ZNF581 (NP_057619.1).
NCBI Gene ID #:
51545
NCBI Official Name:
zinc finger protein 581
NCBI Official Symbol:
ZNF581
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22-24kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9P0T4
User NOte:
Optimal dilutions for each application to be determined by the researcher.