SIAE Antibody

ProSci
Product Code: PSI-14-855
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-855-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SIAE antibody (14-855) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using SIAE antibody (14-855) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Further Information

Additional Names:
AIS6, CSE-C, CSEC, LSE, YSG2, sialate O-acetylesterase, H-Lse, cytosolic sialic acid 9-O-acetylesterase homolog, sialic acid-specific acetylesterase II
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1).
NCBI Gene ID #:
54414
NCBI Official Name:
sialic acid acetylesterase
NCBI Official Symbol:
SIAE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 58kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9HAT2
User NOte:
Optimal dilutions for each application to be determined by the researcher.