DSP Antibody

ProSci
Product Code: PSI-14-900
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-900-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of HepG2 cells, using DSP antibody (14-900) at 1:800 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 3
Immunohistochemistry of paraffin-embedded human gastric cancer using DSP antibody (14-900) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse heart using DSP antibody (14-900) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of HepG2 cells, using DSP antibody (14-900) at 1:800 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human gastric cancer using DSP antibody (14-900) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DSP antibody (14-900) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DSP, DPI, desmoplakin I, desmoplakin, 250kDa paraneoplastic pemphigus antigen, DP, DPII
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2750-2850 of human DSP (NP_004406.2).
NCBI Gene ID #:
1832
NCBI Official Name:
desmoplakin
NCBI Official Symbol:
DSP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 315kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P15924
User NOte:
Optimal dilutions for each application to be determined by the researcher.