HBB Antibody

ProSci
Product Code: PSI-14-908
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-908-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of Raji cells, using HBB antibody (14-908) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts of Raji cells, using HBB antibody (14-908) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
HBB, CD113t-C, Hemoglobin beta, Hemoglobin beta chain, Hemoglobin subunit beta, Beta globin chain, Beta-globin, Hemoglobin, beta
Application Note:
WB: 1:500 - 1:2000
Background:
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human HBB (NP_000509.1).
NCBI Gene ID #:
3043
NCBI Official Name:
hemoglobin, beta
NCBI Official Symbol:
HBB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P68871
User NOte:
Optimal dilutions for each application to be determined by the researcher.