PYCR1 Antibody

ProSci
Product Code: PSI-14-935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-935-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PYCR1 antibody (14-935) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat liver using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using PYCR1 antibody (14-935) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PYCR1 antibody (14-935) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
Pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, PYCR1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
NCBI Gene ID #:
5831
NCBI Official Name:
Pyrroline-5-carboxylate reductase 1, mitochondrial
NCBI Official Symbol:
PYCR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P32322
User NOte:
Optimal dilutions for each application to be determined by the researcher.