SMARCE1 Antibody, KO Validated

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ProSci
Product Code: PSI-14-940
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-14-940-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (14-940) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (14-940) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (14-940).
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Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (14-940). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (14-940) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (14-940) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (14-940) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (14-940).
Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (14-940). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (14-940) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
SMARCE1, BAF57
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
NCBI Gene ID #:
6605
NCBI Official Name:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol:
SMARCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q969G3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.