SOD2 Antibody

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ProSci
Product Code: PSI-14-969
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-969-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SOD2 antibody (14-969) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SOD2 antibody (14-969). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using SOD2 antibody (14-969) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SOD2 antibody (14-969) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SOD2 antibody (14-969). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using SOD2 antibody (14-969) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
superoxide dismutase [Mn], mitochondrial, manganese-containing superoxide dismutase, indophenoloxidase B, Mn superoxide dismutase, MVCD6, MNSOD, IPOB, RP1-56L9.2, superoxide dismutase 2, SOD2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
NCBI Gene ID #:
6648
NCBI Official Name:
superoxide dismutase 2, mitochondrial
NCBI Official Symbol:
SOD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P04179
User NOte:
Optimal dilutions for each application to be determined by the researcher.