CHMP2B Antibody

Contact us
ProSci
Product Code: PSI-14-974
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-974-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using CHMP2B antibody (14-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded human gastric cancer using CHMP2B antibody (14-974) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of MCF-7 cells using CHMP2B antibody (14-974). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using CHMP2B antibody (14-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human gastric cancer using CHMP2B antibody (14-974) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CHMP2B antibody (14-974). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ALS17, CHMP2.5, DMT1, VPS2-2, VPS2B, charged multivesicular body protein 2b, VPS2 homolog B, chromatin modifying protein 2B, vacuolar protein-sorting-associated protein 2-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human CHMP2B (NP_054762.2).
NCBI Gene ID #:
25978
NCBI Official Name:
charged multivesicular body protein 2B
NCBI Official Symbol:
CHMP2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9UQN3
User NOte:
Optimal dilutions for each application to be determined by the researcher.