Cytochrome c Antibody

ProSci
Product Code: PSI-14-986
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-986-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
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Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (14-986) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (14-986) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Cytochrome c antibody (14-986) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (14-986) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
NCBI Gene ID #:
54205
NCBI Official Name:
cytochrome c, somatic
NCBI Official Symbol:
CYCS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P99999
User NOte:
Optimal dilutions for each application to be determined by the researcher.