RAD18 Antibody

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ProSci
Product Code: PSI-14-988
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-988-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RAD18 antibody (14-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
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Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
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Western blot analysis of extracts of various cell lines, using RAD18 antibody (14-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

Documents

Further Information

Additional Names:
RAD18, RNF73
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human RAD18 (NP_064550.3).
NCBI Gene ID #:
56852
NCBI Official Name:
RAD18 homolog (S. cerevisiae)
NCBI Official Symbol:
RAD18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9NS91
User NOte:
Optimal dilutions for each application to be determined by the researcher.