RBBP7 Antibody

ProSci
Product Code: PSI-14-998
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-998-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RBBP7 antibody (14-998) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human gastric cancer using RBBP7 antibody (14-998) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using RBBP7 antibody (14-998). Blue: DAPI for nuclear staining.
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Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody (14-998) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using RBBP7 antibody (14-998) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human gastric cancer using RBBP7 antibody (14-998) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RBBP7 antibody (14-998). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody (14-998) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Further Information

Additional Names:
Histone-binding protein RBBP7, Histone acetyltransferase type B subunit 2, Nucleosome-remodeling factor subunit RBAP46, Retinoblastoma-binding protein 7, RBBP-7, Retinoblastoma-binding protein p46, RBBP7, RBAP46
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

ChIP: 1:50 - 1:200
Background:
This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
NCBI Gene ID #:
5931
NCBI Official Name:
Histone-binding protein RBBP7
NCBI Official Symbol:
RBBP7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q16576
User NOte:
Optimal dilutions for each application to be determined by the researcher.