AK1 Antibody

ProSci
Product Code: PSI-15-002
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-002-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AK1 antibody (15-002) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using AK1 antibody (15-002). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using AK1 antibody (15-002) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody (15-002) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AK1 antibody (15-002). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
AK1, HTL-S-58j
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
NCBI Gene ID #:
203
NCBI Official Name:
adenylate kinase 1
NCBI Official Symbol:
AK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P00568
User NOte:
Optimal dilutions for each application to be determined by the researcher.