ATF3 Antibody

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ProSci
Product Code: PSI-15-007
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-007-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using ATF3 antibody (15-007) at 1:1000 dilution. Raw264.7 cells were treated by LPS (1 ug/ml) at 37°C for 8 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 2
Immunofluorescence analysis of A549 cells using ATF3 antibody (15-007). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ATF3 antibody (15-007) at 1:1000 dilution. Raw264.7 cells were treated by LPS (1 ug/ml) at 37°C for 8 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of A549 cells using ATF3 antibody (15-007). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ATF3,
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
NCBI Gene ID #:
467
NCBI Official Name:
activating transcription factor 3
NCBI Official Symbol:
ATF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Cancer, Transcription
Swissprot #:
P18847
User NOte:
Optimal dilutions for each application to be determined by the researcher.