SERPINH1 Antibody

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ProSci
Product Code: PSI-15-009
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-009-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SERPINH1 antibody (15-009) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse heart using SERPINH1 antibody (15-009) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SERPINH1 antibody (15-009). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SERPINH1 antibody (15-009) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using SERPINH1 antibody (15-009) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SERPINH1 antibody (15-009). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
SERPINH1, CBP1, CBP2, OI10, gp46, AsTP3, HSP47, PIG14, PPROM, RA-A47, SERPINH2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 139-418 of human SERPINH1 (NP_001226.2).
NCBI Gene ID #:
871
NCBI Official Name:
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
NCBI Official Symbol:
SERPINH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P50454
User NOte:
Optimal dilutions for each application to be determined by the researcher.