CD1D Antibody

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ProSci
Product Code: PSI-15-010
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-010-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using CD1D antibody (15-010) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human kidney cancer using CD1D antibody (15-010) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of HeLa cells using CD1D antibody (15-010). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse liver, using CD1D antibody (15-010) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney cancer using CD1D antibody (15-010) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using CD1D antibody (15-010). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CD1D, CD1D antigen, d polypeptide, R3, R3G1, CD1d antigen, CD1d molecule, Thymocyte antigen CD1D
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human CD1D (NP_001757.1).
NCBI Gene ID #:
912
NCBI Official Name:
CD1d molecule
NCBI Official Symbol:
CD1D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Immunology, Stem Cell
Swissprot #:
P15813
User NOte:
Optimal dilutions for each application to be determined by the researcher.