EHHADH Antibody

ProSci
Product Code: PSI-15-015
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-015-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EHHADH antibody (15-015) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human liver damage using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of A549 cells using EHHADH antibody (15-015). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EHHADH antibody (15-015) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EHHADH antibody (15-015). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2).
NCBI Gene ID #:
1962
NCBI Official Name:
Peroxisomal bifunctional enzyme
NCBI Official Symbol:
EHHADH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 79kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q08426
User NOte:
Optimal dilutions for each application to be determined by the researcher.