GSS Antibody

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ProSci
Product Code: PSI-15-022
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-022-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GSS antibody (15-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded mouse lung using GSS antibody (15-022) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of A549 cells using GSS antibody (15-022). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GSS antibody (15-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse lung using GSS antibody (15-022) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using GSS antibody (15-022). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Glutathione synthetase, GSH synthetase, GSH-S, Glutathione synthase, GSS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-474 of human GSS (NP_000169.1).
NCBI Gene ID #:
2937
NCBI Official Name:
Glutathione synthetase
NCBI Official Symbol:
GSS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P48637
User NOte:
Optimal dilutions for each application to be determined by the researcher.