HAS3 Antibody

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ProSci
Product Code: PSI-15-025
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-025-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HAS3 antibody (15-025) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of A549 cells using HAS3 antibody (15-025). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HAS3 antibody (15-025) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using HAS3 antibody (15-025). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3, HA synthase 3, HAS3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 67-281 of human HAS3 (NP_619515.1).
NCBI Gene ID #:
3038
NCBI Official Name:
Hyaluronan synthase 3
NCBI Official Symbol:
HAS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 73kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
O00219
User NOte:
Optimal dilutions for each application to be determined by the researcher.