AGFG1 Antibody

ProSci
Product Code: PSI-15-027
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-027-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AGFG1 antibody (15-027) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of HeLa cells using AGFG1 antibody (15-027). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using AGFG1 antibody (15-027) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using AGFG1 antibody (15-027). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HRB, MGC116938, MGC116940, RAB, RIP, HRB
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).
NCBI Gene ID #:
3267
NCBI Official Name:
ArfGAP with FG repeats 1
NCBI Official Symbol:
AGFG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 58kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
P52594
User NOte:
Optimal dilutions for each application to be determined by the researcher.