LGALS3 Antibody, KO Validated

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ProSci
Product Code: PSI-15-031
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-031-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using LGALS3 antibody (15-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (15-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
3 / 5
Immunofluorescence analysis of C6 cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of L-929 cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of U-2 OS cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using LGALS3 antibody (15-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (15-031) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using LGALS3 antibody (15-031) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
NCBI Gene ID #:
3958
NCBI Official Name:
lectin, galactoside-binding, soluble, 3
NCBI Official Symbol:
LGALS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
P17931
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.