LIG3 Antibody

ProSci
Product Code: PSI-15-032
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-032-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using LIG3 antibody (15-032) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using LIG3 antibody (15-032) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using LIG3 antibody (15-032) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DNA ligase 3, DNA ligase III, LIG2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2).
NCBI Gene ID #:
3980
NCBI Official Name:
ligase III, DNA, ATP-dependent
NCBI Official Symbol:
LIG3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 112kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49916
User NOte:
Optimal dilutions for each application to be determined by the researcher.