MCM5 Antibody

ProSci
Product Code: PSI-15-036
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-036-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MCM5 antibody (15-036) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human lung cancer using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MCM5 antibody (15-036) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human lung cancer using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MCM5, CDC46, P1-CDC46
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
NCBI Gene ID #:
4174
NCBI Official Name:
minichromosome maintenance complex component 5
NCBI Official Symbol:
MCM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P33992
User NOte:
Optimal dilutions for each application to be determined by the researcher.