MDH2 Antibody

ProSci
Product Code: PSI-15-037
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-037-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MDH2 antibody (15-037) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of U2OS cells using MDH2 antibody (15-037). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MDH2 antibody (15-037) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using MDH2 antibody (15-037). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MDH2, M-MDH, MDH, MGC:3559, MOR1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
NCBI Gene ID #:
4191
NCBI Official Name:
malate dehydrogenase 2, NAD (mitochondrial)
NCBI Official Symbol:
MDH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P40926
User NOte:
Optimal dilutions for each application to be determined by the researcher.