PPP4C Antibody

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ProSci
Product Code: PSI-15-048
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-048-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPP4C antibody (15-048) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using PPP4C antibody (15-048). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody (15-048). Western blot was performed from the immunoprecipitate using PPP4C antibody (15-048) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using PPP4C antibody (15-048) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using PPP4C antibody (15-048). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody (15-048). Western blot was performed from the immunoprecipitate using PPP4C antibody (15-048) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, PP4C, PPP4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
NCBI Gene ID #:
5531
NCBI Official Name:
protein phosphatase 4, catalytic subunit
NCBI Official Symbol:
PPP4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P60510
User NOte:
Optimal dilutions for each application to be determined by the researcher.