ST14 Antibody

ProSci
Product Code: PSI-15-062
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-062-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ST14 antibody (15-062) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse liver using ST14 antibody (15-062) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using ST14 antibody (15-062). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ST14 antibody (15-062) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using ST14 antibody (15-062) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using ST14 antibody (15-062). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ST14, HAI, MT-SP1, MTSP-1, MTSP1, PRSS14, SNC19, TADG-15, TADG15, TMPRSS14
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 566-855 of human ST14 (NP_068813.1).
NCBI Gene ID #:
6768
NCBI Official Name:
suppression of tumorigenicity 14 (colon carcinoma)
NCBI Official Symbol:
ST14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y5Y6
User NOte:
Optimal dilutions for each application to be determined by the researcher.