TFAM Antibody

ProSci
Product Code: PSI-15-064
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-064-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TFAM antibody (15-064) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded mouse spleen using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (14-466) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TFAM antibody (15-064) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TFAM antibody (15-064) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (14-466) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Transcription Factor AntibodiesAM, TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).
NCBI Gene ID #:
7019
NCBI Official Name:
transcription factor A, mitochondrial
NCBI Official Symbol:
TFAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction, Transcription
Swissprot #:
Q00059
User NOte:
Optimal dilutions for each application to be determined by the researcher.