DLGAP5 Antibody

ProSci
Product Code: PSI-15-079
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-079-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DLGAP5 antibody (15-079) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of HeLa cells using DLGAP5 antibody (15-079). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DLGAP5 antibody (15-079) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using DLGAP5 antibody (15-079). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DLG7, DLG1, HURP, KIAA0008, DLG7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 547-846 of human DLGAP5 (NP_055565.3).
NCBI Gene ID #:
9787
NCBI Official Name:
discs, large (Drosophila) homolog-associated protein 5
NCBI Official Symbol:
DLGAP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
Q15398
User NOte:
Optimal dilutions for each application to be determined by the researcher.