IGF2BP1 Antibody

ProSci
Product Code: PSI-15-085
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-085-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (15-085) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug IGF2BP1 antibody (15-085). Western blot was performed from the immunoprecipitate using IGF2BP1 antibody (15-085) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (15-085) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug IGF2BP1 antibody (15-085). Western blot was performed from the immunoprecipitate using IGF2BP1 antibody (15-085) at a dilition of 1:1000.

Further Information

Additional Names:
IGF2BP1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 480 to the C-terminus of human IGF2BP1 (NP_006537.3).
NCBI Gene ID #:
10642
NCBI Official Name:
insulin-like growth factor 2 mRNA binding protein 1
NCBI Official Symbol:
IGF2BP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NZI8
User NOte:
Optimal dilutions for each application to be determined by the researcher.