RPH3A Antibody

ProSci
Product Code: PSI-15-087
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-087-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPH3A antibody (15-087) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human liver cancer using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using RPH3A antibody (15-087) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Rabphilin-3A, Exophilin-1, RPH3A, KIAA0985
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2).
NCBI Gene ID #:
22895
NCBI Official Name:
Rabphilin-3A
NCBI Official Symbol:
RPH3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 77kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q9Y2J0
User NOte:
Optimal dilutions for each application to be determined by the researcher.