LSM4 Antibody

ProSci
Product Code: PSI-15-090
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-090-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using LSM4 antibody (15-090) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug LSM4 antibody (15-090). Western blot was performed from the immunoprecipitate using LSM4 antibody (15-090) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using LSM4 antibody (15-090) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug LSM4 antibody (15-090). Western blot was performed from the immunoprecipitate using LSM4 antibody (15-090) at a dilition of 1:1000.

Further Information

Additional Names:
LSM4, YER112W, GRP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).
NCBI Gene ID #:
25804
NCBI Official Name:
LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
NCBI Official Symbol:
LSM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y4Z0
User NOte:
Optimal dilutions for each application to be determined by the researcher.