PSME2 Antibody

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ProSci
Product Code: PSI-15-112
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-112-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PSME2 antibody (15-112) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human gastric cancer using PSME2 antibody (15-112) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PSME2 antibody (15-112) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using PSME2 antibody (15-112) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
PSME2, proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28B, PA28beta, REGbeta, 11S regulator complex beta subunit, MCP activator, 31-kD subunit, activator of multicatalytic protease subunit 2, cell migration-inducing protein 22, proteasome activator 28-beta, proteasome activator hPA28 subunit beta, proteasome activator subunit 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human PSME2 (NP_002809.2).
NCBI Gene ID #:
5721
NCBI Official Name:
proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
NCBI Official Symbol:
PSME2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9UL46
User NOte:
Optimal dilutions for each application to be determined by the researcher.