RPLP0 Antibody

ProSci
Product Code: PSI-15-114
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-114-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPLP0 antibody (15-114) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using RPLP0 antibody (15-114). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RPLP0 antibody (15-114) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using RPLP0 Antibody (15-114) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using RPLP0 antibody (15-114). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RPLP0, L10E, MGC111226, MGC88175, P0, PRLP0, RPP0, LP0
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human RPLP0 (NP_000993.1).
NCBI Gene ID #:
6175
NCBI Official Name:
ribosomal protein, large, P0
NCBI Official Symbol:
RPLP0
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P05388
User NOte:
Optimal dilutions for each application to be determined by the researcher.