TWF2 Antibody

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ProSci
Product Code: PSI-15-123
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-123-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TWF2 antibody (15-123) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody (15-123). Western blot was performed from the immunoprecipitate using TWF2 antibody (15-123) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using TWF2 antibody (15-123) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody (15-123). Western blot was performed from the immunoprecipitate using TWF2 antibody (15-123) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Twinfilin-2, A6-related protein, hA6RP, Protein tyrosine kinase 9-like, Twinfilin-1-like protein, TWF2, PTK9L
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).
NCBI Gene ID #:
11344
NCBI Official Name:
Twinfilin-2
NCBI Official Symbol:
TWF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q6IBS0
User NOte:
Optimal dilutions for each application to be determined by the researcher.