NDUFB7 Antibody

ProSci
Product Code: PSI-15-154
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-154-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDUFB7 antibody (15-154) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of L929 cells using NDUFB7 Polyclonal Antibody (15-154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NDUFB7 antibody (15-154) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of L929 cells using NDUFB7 Polyclonal Antibody (15-154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
B18, CI-B18, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa, NADH-ubiquinone oxidoreductase B18 subunit, cell adhesion protein SQM1, complex I B18 subunit, complex I-B18
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human NDUFB7 (NP_004137.2).
NCBI Gene ID #:
4713
NCBI Official Name:
NADH:ubiquinone oxidoreductase subunit B7
NCBI Official Symbol:
NDUFB7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P17568
User NOte:
Optimal dilutions for each application to be determined by the researcher.