ABCB10 Antibody

ProSci
Product Code: PSI-15-204
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-204-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using ABCB10 antibody (15-204) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 3
Immunofluorescence analysis of C6 cells using ABCB10 antibody (15-204) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using ABCB10 antibody (15-204) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ABCB10 antibody (15-204) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of C6 cells using ABCB10 antibody (15-204) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ABCB10 antibody (15-204) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, sub-family B, ABC-me
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-300 of human ABCB10 (NP_036221.2).
NCBI Gene ID #:
23456
NCBI Official Name:
ATP-binding cassette, sub-family B (MDR/TAP), member 10
NCBI Official Symbol:
ABCB10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9NRK6
User NOte:
Optimal dilutions for each application to be determined by the researcher.