SLC25A27 Antibody

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ProSci
Product Code: PSI-15-224
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-224-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using SLC25A27 Antibody (15-224) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of NIH/3T3 cells using SLC25A27 antibody (15-224) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse brain, using SLC25A27 Antibody (15-224) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using SLC25A27 antibody (15-224) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
UCP4, mitochondrial uncoupling protein 4, UCP 4, uncoupling protein 4
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).
NCBI Gene ID #:
9481
NCBI Official Name:
solute carrier family 25 member 27
NCBI Official Symbol:
SLC25A27
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O95847
User NOte:
Optimal dilutions for each application to be determined by the researcher.