GANAB Antibody

ProSci
Product Code: PSI-15-233
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-233-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GANAB antibody (15-233) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
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Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GANAB antibody (15-233) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GANAB, G2AN, Glucosidase, alpha, Glucosidase, alpha neutral AB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, GLUII, KIAA0088, Neutral AB
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
NCBI Gene ID #:
23193
NCBI Official Name:
glucosidase, alpha; neutral AB
NCBI Official Symbol:
GANAB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 112kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q14697
User NOte:
Optimal dilutions for each application to be determined by the researcher.