Aquaporin 4 Antibody

ProSci
Product Code: PSI-15-269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-269-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (15-269) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).

Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (15-269) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin 4 antibody (15-269) at dilution of 1:50 (40x lens).

Further Information

Additional Names:
MIWC, WCH4, aquaporin-4, aquaporin type4, mercurial-insensitive water channel
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1).
NCBI Gene ID #:
361
NCBI Official Name:
aquaporin 4
NCBI Official Symbol:
AQP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P55087
User NOte:
Optimal dilutions for each application to be determined by the researcher.