MCAM Antibody

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ProSci
Product Code: PSI-15-270
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-270-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MCAM antibody (15-270) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human colon using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using MCAM antibody (15-270) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MCAM antibody (15-270) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MCAM antibody (15-270) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 447-646 of human MCAM (NP_006491.2).
NCBI Gene ID #:
4162
NCBI Official Name:
melanoma cell adhesion molecule
NCBI Official Symbol:
MCAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P43121
User NOte:
Optimal dilutions for each application to be determined by the researcher.