APRT Antibody

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ProSci
Product Code: PSI-15-285
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-285-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using APRT antibody (15-285) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human prostate using APRT Antibody (15-285) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using APRT Antibody (15-285) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using APRT antibody (15-285) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using APRT Antibody (15-285) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using APRT Antibody (15-285) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
APRT, AMP, DKFZp686D13177, MGC125856, MGC125857, MGC129961, APRTD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).
NCBI Gene ID #:
353
NCBI Official Name:
adenine phosphoribosyltransferase
NCBI Official Symbol:
APRT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 19-22kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P07741
User NOte:
Optimal dilutions for each application to be determined by the researcher.