GPC3 Antibody

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ProSci
Product Code: PSI-15-307
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-307-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GPC3 antibody (15-307) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using GPC3 antibody (15-307) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GPC3 antibody (15-307) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
GPC3, SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human GPC3 (NP_004475.1).
NCBI Gene ID #:
2719
NCBI Official Name:
glypican 3
NCBI Official Symbol:
GPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 66kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P51654
User NOte:
Optimal dilutions for each application to be determined by the researcher.