NFIL3 Antibody

ProSci
Product Code: PSI-15-329
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-329-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using NFIL3 antibody (15-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
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Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (15-329). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using NFIL3 antibody (15-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (15-329). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NFIL3, E4BP4, IL3BP1, NFIL3A, NF-IL3A
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2).
NCBI Gene ID #:
4783
NCBI Official Name:
nuclear factor, interleukin 3 regulated
NCBI Official Symbol:
NFIL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity, Transcription
Swissprot #:
Q16649
User NOte:
Optimal dilutions for each application to be determined by the researcher.