NDC80 Antibody

ProSci
Product Code: PSI-15-371
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-371-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDC80 antibody (15-371) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using NDC80 antibody (15-371) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Further Information

Additional Names:
HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC1, HEC-1, HEC, KNTC2
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1).
NCBI Gene ID #:
10403
NCBI Official Name:
NDC80 kinetochore complex component
NCBI Official Symbol:
NDC80
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 74kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
O14777
User NOte:
Optimal dilutions for each application to be determined by the researcher.