NOP58 Antibody

ProSci
Product Code: PSI-15-388
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-388-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NOP58 antibody (15-388) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Western blot analysis of extracts of various cell lines, using NOP58 antibody (15-388) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Further Information

Additional Names:
HSPC12NOP5, NOP5/NOP58, nucleolar protein 58, NOP58 ribonucleoprotein homolog, nucleolar protein 5, nucleolar protein NOP5/NOP58
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-529 of human NOP58 (NP_057018.1).
NCBI Gene ID #:
51602
NCBI Official Name:
NOP58 ribonucleoprotein
NCBI Official Symbol:
NOP58
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y2X3
User NOte:
Optimal dilutions for each application to be determined by the researcher.