ESRRA Antibody

ProSci
Product Code: PSI-15-414
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-414-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ESRRA antibody (15-414) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterus using TFAM antibody (15-064) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ESRRA antibody (15-414) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ESRRA antibody (15-414) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using TFAM antibody (15-064) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Steroid hormone receptor ERR1, Estrogen receptor-like 1, Estrogen-related receptor alpha, ERR-alpha, Nuclear receptor subfamily 3 group B member 1, ESRRA, ERR1, ESRL1, NR3B1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human ESRRA (NP_004442.3).
NCBI Gene ID #:
2101
NCBI Official Name:
Steroid hormone receptor ERR1
NCBI Official Symbol:
ESRRA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction, Transcription
Swissprot #:
P11474
User NOte:
Optimal dilutions for each application to be determined by the researcher.