EPHX2 Antibody

ProSci
Product Code: PSI-15-424
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-424-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EPHX2 antibody (15-424) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using EPHX2 antibody (15-424) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human rectum cancer using EPHX2 antibody (15-424) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using EPHX2 antibody (15-424) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using EPHX2 antibody (15-424) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectum cancer using EPHX2 antibody (15-424) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
EPHX2, epoxide hydrolase 2, cytoplasmic, CEH, SEH, bifunctional epoxide hydrolase 2, epoxide hydratase, epoxide hydrolase 2, cytosolic, epoxide hydrolase, soluble
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 286-555 of human EPHX2 (NP_001970.2).
NCBI Gene ID #:
2053
NCBI Official Name:
epoxide hydrolase 2, cytoplasmic
NCBI Official Symbol:
EPHX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P34913
User NOte:
Optimal dilutions for each application to be determined by the researcher.